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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Ocidental. |
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Data corrente: |
26/10/1998 |
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Data da última atualização: |
13/08/2012 |
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Autoria: |
TEIXEIRA, J. B. |
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Título: |
Development of in vitro techniques of oil palm (Elaeis guineensis Jacq.). |
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Ano de publicação: |
1989 |
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Fonte/Imprenta: |
New Brunswiok: State university of New Jersey, 1989. |
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Páginas: |
161p. |
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Idioma: |
Inglês |
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Notas: |
Ph.D. Thesis. |
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Conteúdo: |
Optimization of specific cultural conditions which enabled establishment of successful protocols leading to somatic embryogenesis from diverse explants of oil palm was undertaken. Callus induction and proliferation were obtained on mature and immature zygotic embryo explants cultured on modified MS medium (Murashige & Skoog, 1962) or modified Y3 medium (Eeuwens, 1978) containing either 2,4-D or picloram, plus activated charcoal. Two types of embryogenic tissue were observed on mature embryo explants: a compact tissue and friable tissue. The compact embryogenic tissue gave rise to lower number of somatic embryos while the friable embryogenic tissue resulted in high frequency embryogenesis. Friable embryogenic cell lines were isolated from mature zygotic embryos. Plantlets were successfully regenerated from both types of embryogenic tissues. Embryogenic cell suspensions were established from friable embryogenic cell lines and fully developed somatic embryos were recovered from these suspension cultures. Callus cultures were established from immature inflorescence tissues cultured on MS medium contening 2,4-D and activated charcoal. In comparison to embryo culture, higher concentrations of auxin and charcoal were required to obtain callus from inflorescence tissues. Moreover, the inflorescence tissues produced callus after 2-3 months in culture as compared to 3 weeks for embryos explants. Somatic embryogenesis was accomplished from immature inflorescence callus. In this case, compact embryogenic tissue was obtained. Fully differentiated embryos were achieved when the embryogenic tissue was transfered to Y3 medium containing NAA and ABA. MenosOptimization of specific cultural conditions which enabled establishment of successful protocols leading to somatic embryogenesis from diverse explants of oil palm was undertaken. Callus induction and proliferation were obtained on mature and immature zygotic embryo explants cultured on modified MS medium (Murashige & Skoog, 1962) or modified Y3 medium (Eeuwens, 1978) containing either 2,4-D or picloram, plus activated charcoal. Two types of embryogenic tissue were observed on mature embryo explants: a compact tissue and friable tissue. The compact embryogenic tissue gave rise to lower number of somatic embryos while the friable embryogenic tissue resulted in high frequency embryogenesis. Friable embryogenic cell lines were isolated from mature zygotic embryos. Plantlets were successfully regenerated from both types of embryogenic tissues. Embryogenic cell suspensions were established from friable embryogenic cell lines and fully developed somatic embryos were recovered from these suspension cultures. Callus cultures were established from immature inflorescence tissues cultured on MS medium contening 2,4-D and activated charcoal. In comparison to embryo culture, higher concentrations of auxin and charcoal were required to obtain callus from inflorescence tissues. Moreover, the inflorescence tissues produced callus after 2-3 months in culture as compared to 3 weeks for embryos explants. Somatic embryogenesis was accomplished from immature inflorescence callus. In this case, ... Mostrar Tudo |
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Palavras-Chave: |
Embryonic development; Estados Unidos da America; Microporpagacao. |
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Thesagro: |
Cultura de Tecido; Cultura In Vitro; Dendê; Elaeis Guineensis; Embriogénese. |
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Thesaurus Nal: |
in vitro culture; micropropagation; New Jersey; tissue culture. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02433nam a2200277 a 4500
001 1666906
005 2012-08-13
008 1989 bl uuuu m 00u1 u #d
100 1 $aTEIXEIRA, J. B.
245 $aDevelopment of in vitro techniques of oil palm (Elaeis guineensis Jacq.).
260 $aNew Brunswiok: State university of New Jersey$c1989
300 $a161p.
500 $aPh.D. Thesis.
520 $aOptimization of specific cultural conditions which enabled establishment of successful protocols leading to somatic embryogenesis from diverse explants of oil palm was undertaken. Callus induction and proliferation were obtained on mature and immature zygotic embryo explants cultured on modified MS medium (Murashige & Skoog, 1962) or modified Y3 medium (Eeuwens, 1978) containing either 2,4-D or picloram, plus activated charcoal. Two types of embryogenic tissue were observed on mature embryo explants: a compact tissue and friable tissue. The compact embryogenic tissue gave rise to lower number of somatic embryos while the friable embryogenic tissue resulted in high frequency embryogenesis. Friable embryogenic cell lines were isolated from mature zygotic embryos. Plantlets were successfully regenerated from both types of embryogenic tissues. Embryogenic cell suspensions were established from friable embryogenic cell lines and fully developed somatic embryos were recovered from these suspension cultures. Callus cultures were established from immature inflorescence tissues cultured on MS medium contening 2,4-D and activated charcoal. In comparison to embryo culture, higher concentrations of auxin and charcoal were required to obtain callus from inflorescence tissues. Moreover, the inflorescence tissues produced callus after 2-3 months in culture as compared to 3 weeks for embryos explants. Somatic embryogenesis was accomplished from immature inflorescence callus. In this case, compact embryogenic tissue was obtained. Fully differentiated embryos were achieved when the embryogenic tissue was transfered to Y3 medium containing NAA and ABA.
650 $ain vitro culture
650 $amicropropagation
650 $aNew Jersey
650 $atissue culture
650 $aCultura de Tecido
650 $aCultura In Vitro
650 $aDendê
650 $aElaeis Guineensis
650 $aEmbriogénese
653 $aEmbryonic development
653 $aEstados Unidos da America
653 $aMicroporpagacao
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Registro original: |
Embrapa Amazônia Ocidental (CPAA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
01/12/2023 |
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Data da última atualização: |
01/12/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 4 |
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Autoria: |
CAVALHEIRO, S. B.; MELO, L. C.; SOUZA, T. L. P. O. de; PEREIRA, H. S. |
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Afiliação: |
SÂMELA BEUTINGER CAVALHEIRO, UNIVERSIDADE FEDERAL DE GOIÁS; LEONARDO CUNHA MELO, CNPAF; THIAGO LIVIO PESSOA OLIV DE SOUZA, CNPAF; HELTON SANTOS PEREIRA, CNPAF. |
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Título: |
Selection of parents and segregating populations of carioca common bean resistant to Fusarium wilt. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Semina Ciências Agrárias, v. 44, n. 2, p. 499-514, mar./abr. 2023. |
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ISSN: |
1679-0359 |
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DOI: |
https://doi.org/10.5433/1679-0359.2023v44n2p499 |
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Idioma: |
Inglês |
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Conteúdo: |
Fusarium wilt (Fusarium oxysporum f. sp. phaseoli) is one of the most common diseases in the common bean (Phaseolus vulgaris) crop, and the most viable alternative for its control is the use of resistant cultivars. However, the number of “carioca” cultivars, the most important commercial group in Brazil, which has a good level of resistance to this disease, is still low. To obtain populations with a higher degree of resistance, seven parents with different levels of resistance to Fusarium and which produce carioca grains were crossed in a complete diallel design. The 21 segregating populations obtained were evaluated in the years/generations 2012/F3, 2013/F4, and 2014/F5 in experiments in Santo Antônio de Goiás, GO, in the winter crop season (sowing in May), together with the check cultivars BRS Notável (resistant) and BRS Cometa (susceptible) in an area naturally infested with Fusarium oxysporum f. sp. phaseoli, under a center pivot irrigation system. Reaction to Fusarium wilt, yield, and 100-seed weight were evaluated. Additive and non-additive effects contributed to control of reaction to Fusarium wilt, yield, and 100-seed weight. The statistically negative general combining ability (ĝ) estimates for BRS Notável (-0.51), CNFC 15872 (-0.20), IPR Juriti (-0.18), and BRSMG Talismã (-0.13) indicate that these parents are recommended for obtaining populations more resistant to Fusarium wilt. Among them, cultivar BRS Notável also showed a statistically positive ĝ estimate for grain yield (87.90). Among the parents recommended for resistance to Fusarium wilt, cultivars IPR Juriti (0.99) and BRS Notável (0.23) are those that most contributed to obtaining populations with larger-sized beans. Cultivar BRS Notável is the most promising parent for participation in new crossing blocks, showing non-zero ĝi estimates for all traits, favoring an increase in resistance to Fusarium wilt, yield, and 100-seed weight. Populations BRS Notável / CNFC 15872, BRS Ametista / BRS Notável, BRS Notável / BRSMG Talismã, and BRS Ametista / BRSMG Talismã showed good mean values for reaction to Fusarium wilt, yield, and 100-seed weight and at least one parent with a good general combining ability estimate for resistance to Fusarium wilt. These four populations are promising for obtaining lines with superior performance for resistance to Fusarium wilt, yield, and larger-sized grains. MenosFusarium wilt (Fusarium oxysporum f. sp. phaseoli) is one of the most common diseases in the common bean (Phaseolus vulgaris) crop, and the most viable alternative for its control is the use of resistant cultivars. However, the number of “carioca” cultivars, the most important commercial group in Brazil, which has a good level of resistance to this disease, is still low. To obtain populations with a higher degree of resistance, seven parents with different levels of resistance to Fusarium and which produce carioca grains were crossed in a complete diallel design. The 21 segregating populations obtained were evaluated in the years/generations 2012/F3, 2013/F4, and 2014/F5 in experiments in Santo Antônio de Goiás, GO, in the winter crop season (sowing in May), together with the check cultivars BRS Notável (resistant) and BRS Cometa (susceptible) in an area naturally infested with Fusarium oxysporum f. sp. phaseoli, under a center pivot irrigation system. Reaction to Fusarium wilt, yield, and 100-seed weight were evaluated. Additive and non-additive effects contributed to control of reaction to Fusarium wilt, yield, and 100-seed weight. The statistically negative general combining ability (ĝ) estimates for BRS Notável (-0.51), CNFC 15872 (-0.20), IPR Juriti (-0.18), and BRSMG Talismã (-0.13) indicate that these parents are recommended for obtaining populations more resistant to Fusarium wilt. Among them, cultivar BRS Notável also showed a statistically positive ĝ estimate for g... Mostrar Tudo |
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Palavras-Chave: |
Dialelo completo; Massa de 100 grãos. |
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Thesagro: |
Feijão; Fusarium Oxysporum; Melhoramento Genético Vegetal; Murcha de Fusarium; Phaseolus Vulgaris; Produtividade. |
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Thesaurus NAL: |
Beans; Plant breeding; Yields. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1159042/1/semina-2023.pdf
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Marc: |
LEADER 03376naa a2200313 a 4500
001 2159042
005 2023-12-01
008 2023 bl uuuu u00u1 u #d
022 $a1679-0359
024 7 $ahttps://doi.org/10.5433/1679-0359.2023v44n2p499$2DOI
100 1 $aCAVALHEIRO, S. B.
245 $aSelection of parents and segregating populations of carioca common bean resistant to Fusarium wilt.$h[electronic resource]
260 $c2023
520 $aFusarium wilt (Fusarium oxysporum f. sp. phaseoli) is one of the most common diseases in the common bean (Phaseolus vulgaris) crop, and the most viable alternative for its control is the use of resistant cultivars. However, the number of “carioca” cultivars, the most important commercial group in Brazil, which has a good level of resistance to this disease, is still low. To obtain populations with a higher degree of resistance, seven parents with different levels of resistance to Fusarium and which produce carioca grains were crossed in a complete diallel design. The 21 segregating populations obtained were evaluated in the years/generations 2012/F3, 2013/F4, and 2014/F5 in experiments in Santo Antônio de Goiás, GO, in the winter crop season (sowing in May), together with the check cultivars BRS Notável (resistant) and BRS Cometa (susceptible) in an area naturally infested with Fusarium oxysporum f. sp. phaseoli, under a center pivot irrigation system. Reaction to Fusarium wilt, yield, and 100-seed weight were evaluated. Additive and non-additive effects contributed to control of reaction to Fusarium wilt, yield, and 100-seed weight. The statistically negative general combining ability (ĝ) estimates for BRS Notável (-0.51), CNFC 15872 (-0.20), IPR Juriti (-0.18), and BRSMG Talismã (-0.13) indicate that these parents are recommended for obtaining populations more resistant to Fusarium wilt. Among them, cultivar BRS Notável also showed a statistically positive ĝ estimate for grain yield (87.90). Among the parents recommended for resistance to Fusarium wilt, cultivars IPR Juriti (0.99) and BRS Notável (0.23) are those that most contributed to obtaining populations with larger-sized beans. Cultivar BRS Notável is the most promising parent for participation in new crossing blocks, showing non-zero ĝi estimates for all traits, favoring an increase in resistance to Fusarium wilt, yield, and 100-seed weight. Populations BRS Notável / CNFC 15872, BRS Ametista / BRS Notável, BRS Notável / BRSMG Talismã, and BRS Ametista / BRSMG Talismã showed good mean values for reaction to Fusarium wilt, yield, and 100-seed weight and at least one parent with a good general combining ability estimate for resistance to Fusarium wilt. These four populations are promising for obtaining lines with superior performance for resistance to Fusarium wilt, yield, and larger-sized grains.
650 $aBeans
650 $aPlant breeding
650 $aYields
650 $aFeijão
650 $aFusarium Oxysporum
650 $aMelhoramento Genético Vegetal
650 $aMurcha de Fusarium
650 $aPhaseolus Vulgaris
650 $aProdutividade
653 $aDialelo completo
653 $aMassa de 100 grãos
700 1 $aMELO, L. C.
700 1 $aSOUZA, T. L. P. O. de
700 1 $aPEREIRA, H. S.
773 $tSemina Ciências Agrárias$gv. 44, n. 2, p. 499-514, mar./abr. 2023.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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